Review



human normal immortalized hepatocyte cell line  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    ATCC human normal immortalized hepatocyte cell line
    Human Normal Immortalized Hepatocyte Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 259 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+normal+hepatocyte+cell+line/THLE-3/pm41990932-57-0-18
    Average 96 stars, based on 259 article reviews
    human normal immortalized hepatocyte cell line - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Expressing:

    Article Title: Upregulation of ubiquitin-conjugating enzyme E2T (UBE2T) predicts poor prognosis and promotes hepatocellular carcinoma progression.
    Article Snippet: Human HCC cell lines (Hep3B, HepG2, SK-help1, and PLHC-1) and human normal hepatocyte cell line (THLE-3) were procured from the American Type Culture Collection (ATCC, Manassas, VA, USA).. All cells were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 100 U/mL penicillin, and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C with 5% CO2. pcDNA3.1-UBE2T, empty vector, UBE2T siRNA (si-UBE2T#1/si-UBE2T#2), scramble siRNA, miR-212-5p mimics, miR-2125p inhibitors and negative controls (mimics NC/ inhibitors NC) were available from Invitrogen (Carlsbad, CA, USA).All cells were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 100 U/mL penicillin, and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C with 5% CO2. pcDNA3.1-UBE2T, empty vector, UBE2T siRNA (si-UBE2T#1/si-UBE2T#2), scramble siRNA, miR-212-5p mimics, miR-2125p..

    Article Title: Upregulation of ubiquitin-conjugating enzyme E2T (UBE2T) predicts poor prognosis and promotes hepatocellular carcinoma progression
    Article Snippet: Human HCC cell lines (Hep3B, HepG2, SK-help1, and PLHC-1) and human normal hepatocyte cell line (THLE-3) were procured from the American Type Culture Collection (ATCC, Manassas, VA, USA).. All cells were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 100 U/mL penicillin, and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C with 5% CO 2 . pcDNA3.1-UBE2T, empty vector, UBE2T siRNA (si-UBE2T#1/si-UBE2T#2), scramble siRNA, miR-212-5p mimics, miR-212-5p inhibitors and negative controls (mimics NC/inhibitors NC) were available from Invitrogen (Carlsbad, CA, USA).All cells were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 100 U/mL penicillin, and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C with 5% CO 2 . pcDNA3.1-UBE2T, empty vector, UBE2T siRNA (si-UBE2T#1/si-UBE2T#2), scramble siRNA, miR-212-5p..

    Article Title: lncRNA PRR34-AS1 promotes HCC development via modulating Wnt/β-catenin pathway by absorbing miR-296-5p and upregulating E2F2 and SOX12
    Article Snippet: Human HCC cell lines (Hep 3B and SK-HEP-1), human normal hepatocyte cell line (THLE-3), and human embryonic kidney cell line (HEK293T) were acquired from American Type Culture Collection (ATCC; Manassas, VA, USA).. Human HCC cell line (Huh7) was derived from Chinese Academy of Sciences (Shanghai, China).Human HCC cell line (Huh7) was derived from Chinese Academy of Sciences (Shanghai, China).

    Quantitative RT-PCR:

    Article Title: Upregulation of ubiquitin-conjugating enzyme E2T (UBE2T) predicts poor prognosis and promotes hepatocellular carcinoma progression.
    Article Snippet: Human HCC cell lines (Hep3B, HepG2, SK-help1, and PLHC-1) and human normal hepatocyte cell line (THLE-3) were procured from the American Type Culture Collection (ATCC, Manassas, VA, USA).. All cells were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 100 U/mL penicillin, and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C with 5% CO2. pcDNA3.1-UBE2T, empty vector, UBE2T siRNA (si-UBE2T#1/si-UBE2T#2), scramble siRNA, miR-212-5p mimics, miR-2125p inhibitors and negative controls (mimics NC/ inhibitors NC) were available from Invitrogen (Carlsbad, CA, USA).All cells were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 100 U/mL penicillin, and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C with 5% CO2. pcDNA3.1-UBE2T, empty vector, UBE2T siRNA (si-UBE2T#1/si-UBE2T#2), scramble siRNA, miR-212-5p mimics, miR-2125p..

    Article Title: Upregulation of ubiquitin-conjugating enzyme E2T (UBE2T) predicts poor prognosis and promotes hepatocellular carcinoma progression
    Article Snippet: Human HCC cell lines (Hep3B, HepG2, SK-help1, and PLHC-1) and human normal hepatocyte cell line (THLE-3) were procured from the American Type Culture Collection (ATCC, Manassas, VA, USA).. All cells were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 100 U/mL penicillin, and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C with 5% CO 2 . pcDNA3.1-UBE2T, empty vector, UBE2T siRNA (si-UBE2T#1/si-UBE2T#2), scramble siRNA, miR-212-5p mimics, miR-212-5p inhibitors and negative controls (mimics NC/inhibitors NC) were available from Invitrogen (Carlsbad, CA, USA).All cells were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 100 U/mL penicillin, and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C with 5% CO 2 . pcDNA3.1-UBE2T, empty vector, UBE2T siRNA (si-UBE2T#1/si-UBE2T#2), scramble siRNA, miR-212-5p..

    Article Title: lncRNA PRR34-AS1 promotes HCC development via modulating Wnt/β-catenin pathway by absorbing miR-296-5p and upregulating E2F2 and SOX12
    Article Snippet: Human HCC cell lines (Hep 3B and SK-HEP-1), human normal hepatocyte cell line (THLE-3), and human embryonic kidney cell line (HEK293T) were acquired from American Type Culture Collection (ATCC; Manassas, VA, USA).. Human HCC cell line (Huh7) was derived from Chinese Academy of Sciences (Shanghai, China).Human HCC cell line (Huh7) was derived from Chinese Academy of Sciences (Shanghai, China).

    Fractionation:

    Article Title: Upregulation of ubiquitin-conjugating enzyme E2T (UBE2T) predicts poor prognosis and promotes hepatocellular carcinoma progression.
    Article Snippet: Human HCC cell lines (Hep3B, HepG2, SK-help1, and PLHC-1) and human normal hepatocyte cell line (THLE-3) were procured from the American Type Culture Collection (ATCC, Manassas, VA, USA).. All cells were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 100 U/mL penicillin, and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C with 5% CO2. pcDNA3.1-UBE2T, empty vector, UBE2T siRNA (si-UBE2T#1/si-UBE2T#2), scramble siRNA, miR-212-5p mimics, miR-2125p inhibitors and negative controls (mimics NC/ inhibitors NC) were available from Invitrogen (Carlsbad, CA, USA).All cells were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 100 U/mL penicillin, and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C with 5% CO2. pcDNA3.1-UBE2T, empty vector, UBE2T siRNA (si-UBE2T#1/si-UBE2T#2), scramble siRNA, miR-212-5p mimics, miR-2125p..

    Article Title: Upregulation of ubiquitin-conjugating enzyme E2T (UBE2T) predicts poor prognosis and promotes hepatocellular carcinoma progression
    Article Snippet: Human HCC cell lines (Hep3B, HepG2, SK-help1, and PLHC-1) and human normal hepatocyte cell line (THLE-3) were procured from the American Type Culture Collection (ATCC, Manassas, VA, USA).. All cells were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 100 U/mL penicillin, and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C with 5% CO 2 . pcDNA3.1-UBE2T, empty vector, UBE2T siRNA (si-UBE2T#1/si-UBE2T#2), scramble siRNA, miR-212-5p mimics, miR-212-5p inhibitors and negative controls (mimics NC/inhibitors NC) were available from Invitrogen (Carlsbad, CA, USA).All cells were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 100 U/mL penicillin, and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C with 5% CO 2 . pcDNA3.1-UBE2T, empty vector, UBE2T siRNA (si-UBE2T#1/si-UBE2T#2), scramble siRNA, miR-212-5p..

    Article Title: lncRNA PRR34-AS1 promotes HCC development via modulating Wnt/β-catenin pathway by absorbing miR-296-5p and upregulating E2F2 and SOX12
    Article Snippet: Human HCC cell lines (Hep 3B and SK-HEP-1), human normal hepatocyte cell line (THLE-3), and human embryonic kidney cell line (HEK293T) were acquired from American Type Culture Collection (ATCC; Manassas, VA, USA).. Human HCC cell line (Huh7) was derived from Chinese Academy of Sciences (Shanghai, China).Human HCC cell line (Huh7) was derived from Chinese Academy of Sciences (Shanghai, China).

    Knockdown:

    Article Title: Upregulation of ubiquitin-conjugating enzyme E2T (UBE2T) predicts poor prognosis and promotes hepatocellular carcinoma progression.
    Article Snippet: Human HCC cell lines (Hep3B, HepG2, SK-help1, and PLHC-1) and human normal hepatocyte cell line (THLE-3) were procured from the American Type Culture Collection (ATCC, Manassas, VA, USA).. All cells were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 100 U/mL penicillin, and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C with 5% CO2. pcDNA3.1-UBE2T, empty vector, UBE2T siRNA (si-UBE2T#1/si-UBE2T#2), scramble siRNA, miR-212-5p mimics, miR-2125p inhibitors and negative controls (mimics NC/ inhibitors NC) were available from Invitrogen (Carlsbad, CA, USA).All cells were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 100 U/mL penicillin, and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C with 5% CO2. pcDNA3.1-UBE2T, empty vector, UBE2T siRNA (si-UBE2T#1/si-UBE2T#2), scramble siRNA, miR-212-5p mimics, miR-2125p..

    Article Title: Upregulation of ubiquitin-conjugating enzyme E2T (UBE2T) predicts poor prognosis and promotes hepatocellular carcinoma progression
    Article Snippet: Human HCC cell lines (Hep3B, HepG2, SK-help1, and PLHC-1) and human normal hepatocyte cell line (THLE-3) were procured from the American Type Culture Collection (ATCC, Manassas, VA, USA).. All cells were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 100 U/mL penicillin, and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C with 5% CO 2 . pcDNA3.1-UBE2T, empty vector, UBE2T siRNA (si-UBE2T#1/si-UBE2T#2), scramble siRNA, miR-212-5p mimics, miR-212-5p inhibitors and negative controls (mimics NC/inhibitors NC) were available from Invitrogen (Carlsbad, CA, USA).All cells were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 100 U/mL penicillin, and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C with 5% CO 2 . pcDNA3.1-UBE2T, empty vector, UBE2T siRNA (si-UBE2T#1/si-UBE2T#2), scramble siRNA, miR-212-5p..

    Article Title: lncRNA PRR34-AS1 promotes HCC development via modulating Wnt/β-catenin pathway by absorbing miR-296-5p and upregulating E2F2 and SOX12
    Article Snippet: Human HCC cell lines (Hep 3B and SK-HEP-1), human normal hepatocyte cell line (THLE-3), and human embryonic kidney cell line (HEK293T) were acquired from American Type Culture Collection (ATCC; Manassas, VA, USA).. Human HCC cell line (Huh7) was derived from Chinese Academy of Sciences (Shanghai, China).Human HCC cell line (Huh7) was derived from Chinese Academy of Sciences (Shanghai, China).

    Over Expression:

    Article Title: Upregulation of ubiquitin-conjugating enzyme E2T (UBE2T) predicts poor prognosis and promotes hepatocellular carcinoma progression.
    Article Snippet: Human HCC cell lines (Hep3B, HepG2, SK-help1, and PLHC-1) and human normal hepatocyte cell line (THLE-3) were procured from the American Type Culture Collection (ATCC, Manassas, VA, USA).. All cells were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 100 U/mL penicillin, and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C with 5% CO2. pcDNA3.1-UBE2T, empty vector, UBE2T siRNA (si-UBE2T#1/si-UBE2T#2), scramble siRNA, miR-212-5p mimics, miR-2125p inhibitors and negative controls (mimics NC/ inhibitors NC) were available from Invitrogen (Carlsbad, CA, USA).All cells were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 100 U/mL penicillin, and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C with 5% CO2. pcDNA3.1-UBE2T, empty vector, UBE2T siRNA (si-UBE2T#1/si-UBE2T#2), scramble siRNA, miR-212-5p mimics, miR-2125p..

    Article Title: Upregulation of ubiquitin-conjugating enzyme E2T (UBE2T) predicts poor prognosis and promotes hepatocellular carcinoma progression
    Article Snippet: Human HCC cell lines (Hep3B, HepG2, SK-help1, and PLHC-1) and human normal hepatocyte cell line (THLE-3) were procured from the American Type Culture Collection (ATCC, Manassas, VA, USA).. All cells were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 100 U/mL penicillin, and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C with 5% CO 2 . pcDNA3.1-UBE2T, empty vector, UBE2T siRNA (si-UBE2T#1/si-UBE2T#2), scramble siRNA, miR-212-5p mimics, miR-212-5p inhibitors and negative controls (mimics NC/inhibitors NC) were available from Invitrogen (Carlsbad, CA, USA).All cells were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 100 U/mL penicillin, and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C with 5% CO 2 . pcDNA3.1-UBE2T, empty vector, UBE2T siRNA (si-UBE2T#1/si-UBE2T#2), scramble siRNA, miR-212-5p..

    Article Title: lncRNA PRR34-AS1 promotes HCC development via modulating Wnt/β-catenin pathway by absorbing miR-296-5p and upregulating E2F2 and SOX12
    Article Snippet: Human HCC cell lines (Hep 3B and SK-HEP-1), human normal hepatocyte cell line (THLE-3), and human embryonic kidney cell line (HEK293T) were acquired from American Type Culture Collection (ATCC; Manassas, VA, USA).. Human HCC cell line (Huh7) was derived from Chinese Academy of Sciences (Shanghai, China).Human HCC cell line (Huh7) was derived from Chinese Academy of Sciences (Shanghai, China).

    Binding Assay:

    Article Title: Upregulation of ubiquitin-conjugating enzyme E2T (UBE2T) predicts poor prognosis and promotes hepatocellular carcinoma progression.
    Article Snippet: Human HCC cell lines (Hep3B, HepG2, SK-help1, and PLHC-1) and human normal hepatocyte cell line (THLE-3) were procured from the American Type Culture Collection (ATCC, Manassas, VA, USA).. All cells were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 100 U/mL penicillin, and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C with 5% CO2. pcDNA3.1-UBE2T, empty vector, UBE2T siRNA (si-UBE2T#1/si-UBE2T#2), scramble siRNA, miR-212-5p mimics, miR-2125p inhibitors and negative controls (mimics NC/ inhibitors NC) were available from Invitrogen (Carlsbad, CA, USA).All cells were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 100 U/mL penicillin, and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C with 5% CO2. pcDNA3.1-UBE2T, empty vector, UBE2T siRNA (si-UBE2T#1/si-UBE2T#2), scramble siRNA, miR-212-5p mimics, miR-2125p..

    Article Title: Upregulation of ubiquitin-conjugating enzyme E2T (UBE2T) predicts poor prognosis and promotes hepatocellular carcinoma progression
    Article Snippet: Human HCC cell lines (Hep3B, HepG2, SK-help1, and PLHC-1) and human normal hepatocyte cell line (THLE-3) were procured from the American Type Culture Collection (ATCC, Manassas, VA, USA).. All cells were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 100 U/mL penicillin, and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C with 5% CO 2 . pcDNA3.1-UBE2T, empty vector, UBE2T siRNA (si-UBE2T#1/si-UBE2T#2), scramble siRNA, miR-212-5p mimics, miR-212-5p inhibitors and negative controls (mimics NC/inhibitors NC) were available from Invitrogen (Carlsbad, CA, USA).All cells were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 100 U/mL penicillin, and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C with 5% CO 2 . pcDNA3.1-UBE2T, empty vector, UBE2T siRNA (si-UBE2T#1/si-UBE2T#2), scramble siRNA, miR-212-5p..

    Article Title: lncRNA PRR34-AS1 promotes HCC development via modulating Wnt/β-catenin pathway by absorbing miR-296-5p and upregulating E2F2 and SOX12
    Article Snippet: Human HCC cell lines (Hep 3B and SK-HEP-1), human normal hepatocyte cell line (THLE-3), and human embryonic kidney cell line (HEK293T) were acquired from American Type Culture Collection (ATCC; Manassas, VA, USA).. Human HCC cell line (Huh7) was derived from Chinese Academy of Sciences (Shanghai, China).Human HCC cell line (Huh7) was derived from Chinese Academy of Sciences (Shanghai, China).

    Luciferase:

    Article Title: Upregulation of ubiquitin-conjugating enzyme E2T (UBE2T) predicts poor prognosis and promotes hepatocellular carcinoma progression.
    Article Snippet: Human HCC cell lines (Hep3B, HepG2, SK-help1, and PLHC-1) and human normal hepatocyte cell line (THLE-3) were procured from the American Type Culture Collection (ATCC, Manassas, VA, USA).. All cells were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 100 U/mL penicillin, and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C with 5% CO2. pcDNA3.1-UBE2T, empty vector, UBE2T siRNA (si-UBE2T#1/si-UBE2T#2), scramble siRNA, miR-212-5p mimics, miR-2125p inhibitors and negative controls (mimics NC/ inhibitors NC) were available from Invitrogen (Carlsbad, CA, USA).All cells were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 100 U/mL penicillin, and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C with 5% CO2. pcDNA3.1-UBE2T, empty vector, UBE2T siRNA (si-UBE2T#1/si-UBE2T#2), scramble siRNA, miR-212-5p mimics, miR-2125p..

    Article Title: Upregulation of ubiquitin-conjugating enzyme E2T (UBE2T) predicts poor prognosis and promotes hepatocellular carcinoma progression
    Article Snippet: Human HCC cell lines (Hep3B, HepG2, SK-help1, and PLHC-1) and human normal hepatocyte cell line (THLE-3) were procured from the American Type Culture Collection (ATCC, Manassas, VA, USA).. All cells were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 100 U/mL penicillin, and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C with 5% CO 2 . pcDNA3.1-UBE2T, empty vector, UBE2T siRNA (si-UBE2T#1/si-UBE2T#2), scramble siRNA, miR-212-5p mimics, miR-212-5p inhibitors and negative controls (mimics NC/inhibitors NC) were available from Invitrogen (Carlsbad, CA, USA).All cells were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 100 U/mL penicillin, and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C with 5% CO 2 . pcDNA3.1-UBE2T, empty vector, UBE2T siRNA (si-UBE2T#1/si-UBE2T#2), scramble siRNA, miR-212-5p..

    Article Title: lncRNA PRR34-AS1 promotes HCC development via modulating Wnt/β-catenin pathway by absorbing miR-296-5p and upregulating E2F2 and SOX12
    Article Snippet: Human HCC cell lines (Hep 3B and SK-HEP-1), human normal hepatocyte cell line (THLE-3), and human embryonic kidney cell line (HEK293T) were acquired from American Type Culture Collection (ATCC; Manassas, VA, USA).. Human HCC cell line (Huh7) was derived from Chinese Academy of Sciences (Shanghai, China).Human HCC cell line (Huh7) was derived from Chinese Academy of Sciences (Shanghai, China).

    Activity Assay:

    Article Title: Upregulation of ubiquitin-conjugating enzyme E2T (UBE2T) predicts poor prognosis and promotes hepatocellular carcinoma progression.
    Article Snippet: Human HCC cell lines (Hep3B, HepG2, SK-help1, and PLHC-1) and human normal hepatocyte cell line (THLE-3) were procured from the American Type Culture Collection (ATCC, Manassas, VA, USA).. All cells were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 100 U/mL penicillin, and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C with 5% CO2. pcDNA3.1-UBE2T, empty vector, UBE2T siRNA (si-UBE2T#1/si-UBE2T#2), scramble siRNA, miR-212-5p mimics, miR-2125p inhibitors and negative controls (mimics NC/ inhibitors NC) were available from Invitrogen (Carlsbad, CA, USA).All cells were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 100 U/mL penicillin, and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C with 5% CO2. pcDNA3.1-UBE2T, empty vector, UBE2T siRNA (si-UBE2T#1/si-UBE2T#2), scramble siRNA, miR-212-5p mimics, miR-2125p..

    Article Title: Upregulation of ubiquitin-conjugating enzyme E2T (UBE2T) predicts poor prognosis and promotes hepatocellular carcinoma progression
    Article Snippet: Human HCC cell lines (Hep3B, HepG2, SK-help1, and PLHC-1) and human normal hepatocyte cell line (THLE-3) were procured from the American Type Culture Collection (ATCC, Manassas, VA, USA).. All cells were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 100 U/mL penicillin, and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C with 5% CO 2 . pcDNA3.1-UBE2T, empty vector, UBE2T siRNA (si-UBE2T#1/si-UBE2T#2), scramble siRNA, miR-212-5p mimics, miR-212-5p inhibitors and negative controls (mimics NC/inhibitors NC) were available from Invitrogen (Carlsbad, CA, USA).All cells were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 100 U/mL penicillin, and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) at 37°C with 5% CO 2 . pcDNA3.1-UBE2T, empty vector, UBE2T siRNA (si-UBE2T#1/si-UBE2T#2), scramble siRNA, miR-212-5p..

    Article Title: lncRNA PRR34-AS1 promotes HCC development via modulating Wnt/β-catenin pathway by absorbing miR-296-5p and upregulating E2F2 and SOX12
    Article Snippet: Human HCC cell lines (Hep 3B and SK-HEP-1), human normal hepatocyte cell line (THLE-3), and human embryonic kidney cell line (HEK293T) were acquired from American Type Culture Collection (ATCC; Manassas, VA, USA).. Human HCC cell line (Huh7) was derived from Chinese Academy of Sciences (Shanghai, China).Human HCC cell line (Huh7) was derived from Chinese Academy of Sciences (Shanghai, China).



    Similar Products

    human normal immortalized hepatocyte cell line  (ATCC)
    96
    ATCC human normal immortalized hepatocyte cell line
    Human Normal Immortalized Hepatocyte Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+normal+hepatocyte+cell+line/THLE-3/pm41990932-57-0-18
    Average 96 stars, based on 1 article reviews
    human normal immortalized hepatocyte cell line - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    human normal hepatocyte cell line hepatocytes  (ATCC)
    96
    ATCC human normal hepatocyte cell line hepatocytes
    Human Normal Hepatocyte Cell Line Hepatocytes, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+normal+hepatocyte+cell+line/THLE-3/pm40593862-222-0-11
    Average 96 stars, based on 1 article reviews
    human normal hepatocyte cell line hepatocytes - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    hl-7702 cells (human normal hepatocyte cell line)  (iCell Bioscience Inc)
    90
    iCell Bioscience Inc hl-7702 cells (human normal hepatocyte cell line)
    Hl 7702 Cells (Human Normal Hepatocyte Cell Line), supplied by iCell Bioscience Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+normal+hepatocyte+cell+line/l02+cells/pm39884472-55-0-26
    Average 90 stars, based on 1 article reviews
    hl-7702 cells (human normal hepatocyte cell line) - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    human normal hepatocyte cell line lo2  (Servicebio Inc)
    90
    Servicebio Inc human normal hepatocyte cell line lo2
    Analysis of the expression of POC1A in various classifications of HCC patients, as well as in HCC and liver cancer cell lines. (A) Comparison of POC1A between cancer tissue samples and normal tissue samples. (B) Comparison of the expression of POC1A in different genders. (C) Comparison of the expression of POC1A in different age groups. (D) The expression levels of POC1A were all higher in the Caucasian, African-American, and Asian HCC patients than the normal patients. The expression of POC1A was significantly higher in LIHC tissue samples from Asian patients than LIHC tissue samples from Caucasian patients. (E) The expression of POC1A in individual cancer stages. (F) The expression of POC1A in different transistor grades. (G) The expression of POC1A in tumor (T) stage. (H) The expression of POC1A in node (N) stage. (I) The expression of POC1A in metastasis (M) stage. (J) Analysis of POC1A expression in normal liver tissue and cancer tissue samples from patients with hepatocellular carcinoma using immunohistochemical methods in the Human Protein Atlas database. Liver sample: https://www.proteinatlas.org/ENSG00000164087-POC1A/tissue/liver#img , hepatocellular samples: https://www.proteinatlas.org/ENSG00000164087-POC1A/pathology/liver+cancer#img . Scale bar: 100 µm. (K) Analysis of POC1A mRNA expression in <t>LO2</t> and HepG2 cell lines by qPCR assay. *, P<0.05; **, P<0.01; -: no significance. TCGA, The Cancer Genome Atlas; HCC, hepatocellular carcinoma; LIHC, liver hepatocellular carcinoma; mRNA, messenger RNA; qPCR, quantitative polymerase chain reaction; POC1A , POC1 centriolar protein A.
    Human Normal Hepatocyte Cell Line Lo2, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+normal+hepatocyte+cell+line/human+normal+hepatocyte+cell+line+lo2/pmc11483421-94-2-7
    Average 90 stars, based on 1 article reviews
    human normal hepatocyte cell line lo2 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    human normal hepatocyte cell line hl7702  (Beyotime)
    90
    Beyotime human normal hepatocyte cell line hl7702
    Analysis of the expression of POC1A in various classifications of HCC patients, as well as in HCC and liver cancer cell lines. (A) Comparison of POC1A between cancer tissue samples and normal tissue samples. (B) Comparison of the expression of POC1A in different genders. (C) Comparison of the expression of POC1A in different age groups. (D) The expression levels of POC1A were all higher in the Caucasian, African-American, and Asian HCC patients than the normal patients. The expression of POC1A was significantly higher in LIHC tissue samples from Asian patients than LIHC tissue samples from Caucasian patients. (E) The expression of POC1A in individual cancer stages. (F) The expression of POC1A in different transistor grades. (G) The expression of POC1A in tumor (T) stage. (H) The expression of POC1A in node (N) stage. (I) The expression of POC1A in metastasis (M) stage. (J) Analysis of POC1A expression in normal liver tissue and cancer tissue samples from patients with hepatocellular carcinoma using immunohistochemical methods in the Human Protein Atlas database. Liver sample: https://www.proteinatlas.org/ENSG00000164087-POC1A/tissue/liver#img , hepatocellular samples: https://www.proteinatlas.org/ENSG00000164087-POC1A/pathology/liver+cancer#img . Scale bar: 100 µm. (K) Analysis of POC1A mRNA expression in <t>LO2</t> and HepG2 cell lines by qPCR assay. *, P<0.05; **, P<0.01; -: no significance. TCGA, The Cancer Genome Atlas; HCC, hepatocellular carcinoma; LIHC, liver hepatocellular carcinoma; mRNA, messenger RNA; qPCR, quantitative polymerase chain reaction; POC1A , POC1 centriolar protein A.
    Human Normal Hepatocyte Cell Line Hl7702, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+normal+hepatocyte+cell+line/trizol+reagent/pmc11483456-92-0-16
    Average 90 stars, based on 1 article reviews
    human normal hepatocyte cell line hl7702 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    normal human hepatocytes cell line wrl68  (iCell Bioscience Inc)
    90
    iCell Bioscience Inc normal human hepatocytes cell line wrl68
    Analysis of the expression of POC1A in various classifications of HCC patients, as well as in HCC and liver cancer cell lines. (A) Comparison of POC1A between cancer tissue samples and normal tissue samples. (B) Comparison of the expression of POC1A in different genders. (C) Comparison of the expression of POC1A in different age groups. (D) The expression levels of POC1A were all higher in the Caucasian, African-American, and Asian HCC patients than the normal patients. The expression of POC1A was significantly higher in LIHC tissue samples from Asian patients than LIHC tissue samples from Caucasian patients. (E) The expression of POC1A in individual cancer stages. (F) The expression of POC1A in different transistor grades. (G) The expression of POC1A in tumor (T) stage. (H) The expression of POC1A in node (N) stage. (I) The expression of POC1A in metastasis (M) stage. (J) Analysis of POC1A expression in normal liver tissue and cancer tissue samples from patients with hepatocellular carcinoma using immunohistochemical methods in the Human Protein Atlas database. Liver sample: https://www.proteinatlas.org/ENSG00000164087-POC1A/tissue/liver#img , hepatocellular samples: https://www.proteinatlas.org/ENSG00000164087-POC1A/pathology/liver+cancer#img . Scale bar: 100 µm. (K) Analysis of POC1A mRNA expression in <t>LO2</t> and HepG2 cell lines by qPCR assay. *, P<0.05; **, P<0.01; -: no significance. TCGA, The Cancer Genome Atlas; HCC, hepatocellular carcinoma; LIHC, liver hepatocellular carcinoma; mRNA, messenger RNA; qPCR, quantitative polymerase chain reaction; POC1A , POC1 centriolar protein A.
    Normal Human Hepatocytes Cell Line Wrl68, supplied by iCell Bioscience Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+normal+hepatocyte+cell+line/normal+human+hepatocytes+cell+line+wrl68/pm39029315-49-0-9
    Average 90 stars, based on 1 article reviews
    normal human hepatocytes cell line wrl68 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    human normal hepatocyte cell line l-o2  (Procell Inc)
    90
    Procell Inc human normal hepatocyte cell line l-o2
    Analysis of the expression of POC1A in various classifications of HCC patients, as well as in HCC and liver cancer cell lines. (A) Comparison of POC1A between cancer tissue samples and normal tissue samples. (B) Comparison of the expression of POC1A in different genders. (C) Comparison of the expression of POC1A in different age groups. (D) The expression levels of POC1A were all higher in the Caucasian, African-American, and Asian HCC patients than the normal patients. The expression of POC1A was significantly higher in LIHC tissue samples from Asian patients than LIHC tissue samples from Caucasian patients. (E) The expression of POC1A in individual cancer stages. (F) The expression of POC1A in different transistor grades. (G) The expression of POC1A in tumor (T) stage. (H) The expression of POC1A in node (N) stage. (I) The expression of POC1A in metastasis (M) stage. (J) Analysis of POC1A expression in normal liver tissue and cancer tissue samples from patients with hepatocellular carcinoma using immunohistochemical methods in the Human Protein Atlas database. Liver sample: https://www.proteinatlas.org/ENSG00000164087-POC1A/tissue/liver#img , hepatocellular samples: https://www.proteinatlas.org/ENSG00000164087-POC1A/pathology/liver+cancer#img . Scale bar: 100 µm. (K) Analysis of POC1A mRNA expression in <t>LO2</t> and HepG2 cell lines by qPCR assay. *, P<0.05; **, P<0.01; -: no significance. TCGA, The Cancer Genome Atlas; HCC, hepatocellular carcinoma; LIHC, liver hepatocellular carcinoma; mRNA, messenger RNA; qPCR, quantitative polymerase chain reaction; POC1A , POC1 centriolar protein A.
    Human Normal Hepatocyte Cell Line L O2, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+normal+hepatocyte+cell+line/human+normal+liver+cell+line+lo2/pmc11046247-37-0-18
    Average 90 stars, based on 1 article reviews
    human normal hepatocyte cell line l-o2 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Analysis of the expression of POC1A in various classifications of HCC patients, as well as in HCC and liver cancer cell lines. (A) Comparison of POC1A between cancer tissue samples and normal tissue samples. (B) Comparison of the expression of POC1A in different genders. (C) Comparison of the expression of POC1A in different age groups. (D) The expression levels of POC1A were all higher in the Caucasian, African-American, and Asian HCC patients than the normal patients. The expression of POC1A was significantly higher in LIHC tissue samples from Asian patients than LIHC tissue samples from Caucasian patients. (E) The expression of POC1A in individual cancer stages. (F) The expression of POC1A in different transistor grades. (G) The expression of POC1A in tumor (T) stage. (H) The expression of POC1A in node (N) stage. (I) The expression of POC1A in metastasis (M) stage. (J) Analysis of POC1A expression in normal liver tissue and cancer tissue samples from patients with hepatocellular carcinoma using immunohistochemical methods in the Human Protein Atlas database. Liver sample: https://www.proteinatlas.org/ENSG00000164087-POC1A/tissue/liver#img , hepatocellular samples: https://www.proteinatlas.org/ENSG00000164087-POC1A/pathology/liver+cancer#img . Scale bar: 100 µm. (K) Analysis of POC1A mRNA expression in LO2 and HepG2 cell lines by qPCR assay. *, P<0.05; **, P<0.01; -: no significance. TCGA, The Cancer Genome Atlas; HCC, hepatocellular carcinoma; LIHC, liver hepatocellular carcinoma; mRNA, messenger RNA; qPCR, quantitative polymerase chain reaction; POC1A , POC1 centriolar protein A.

    Journal: Translational Cancer Research

    Article Title: Analysis of the role of POC1A in the development and progression of hepatocellular carcinoma

    doi: 10.21037/tcr-23-2398

    Figure Lengend Snippet: Analysis of the expression of POC1A in various classifications of HCC patients, as well as in HCC and liver cancer cell lines. (A) Comparison of POC1A between cancer tissue samples and normal tissue samples. (B) Comparison of the expression of POC1A in different genders. (C) Comparison of the expression of POC1A in different age groups. (D) The expression levels of POC1A were all higher in the Caucasian, African-American, and Asian HCC patients than the normal patients. The expression of POC1A was significantly higher in LIHC tissue samples from Asian patients than LIHC tissue samples from Caucasian patients. (E) The expression of POC1A in individual cancer stages. (F) The expression of POC1A in different transistor grades. (G) The expression of POC1A in tumor (T) stage. (H) The expression of POC1A in node (N) stage. (I) The expression of POC1A in metastasis (M) stage. (J) Analysis of POC1A expression in normal liver tissue and cancer tissue samples from patients with hepatocellular carcinoma using immunohistochemical methods in the Human Protein Atlas database. Liver sample: https://www.proteinatlas.org/ENSG00000164087-POC1A/tissue/liver#img , hepatocellular samples: https://www.proteinatlas.org/ENSG00000164087-POC1A/pathology/liver+cancer#img . Scale bar: 100 µm. (K) Analysis of POC1A mRNA expression in LO2 and HepG2 cell lines by qPCR assay. *, P<0.05; **, P<0.01; -: no significance. TCGA, The Cancer Genome Atlas; HCC, hepatocellular carcinoma; LIHC, liver hepatocellular carcinoma; mRNA, messenger RNA; qPCR, quantitative polymerase chain reaction; POC1A , POC1 centriolar protein A.

    Article Snippet: The human normal hepatocyte cell line LO2 (Servicebio Technology Co., Ltd., Wuhan, China) was cultured in Roswell Park Memorial Institute 1640 medium, and the hepatoma cell line HepG2 (Procell Life Science & Technology Co., Ltd., Wuhan, China) was cultured in Dulbecco’s Modified Eagle Medium (Hyclone, Thermo Fisher Scientific Inc., Waltham, MA), supplemented with 10% fetal bovine serum and antibiotics (10,000 U/mL penicillin and 10 mg/mL streptomycin) (Procell Life Science & Technology Co., Ltd.).

    Techniques: Expressing, Comparison, Immunohistochemical staining, Real-time Polymerase Chain Reaction